Reciprocal Packaging of the Main Structural Proteins of Type 1 Fimbriae and Flagella in the Outer Membrane Vesicles of “Wild Type” Escherichia coli Strains

Fundamental aspects of outer membrane vesicle (OMV) biogenesis and the engineering producer strains have been major research foci for many in recent years. The focus this study was OMV production a variety Escherichia coli including wild type (WT) (K12 BW25113), mutants (from Keio collection) proprietary [BL21 BL21 (DE3)] strains. present investigated proteome prospective mechanism that underpinned key finding dominant protein E. K-12 WT OMVs fimbrial monomer (FimA) (a polymerizable which is structural from Type 1 fimbriae are made). However, mutations genes involved biosynthesis (Δ fimA , B C F ) resulted packaging flagella (FliC) (the flagella) into instead FimA. Other fimE, G, H, I Δ lrhA –a transcriptional regulator fimbriation biosynthesis) lead to both FimA Flagellin OMVs. In majority instances shown within research, considered where appendages or temporally co-expressed throughout growth curve as previously literature. hypothesis, proposed supported paper, vesicular reciprocally regulated with FliC but abrogated range mutated, non-WT We also demonstrate, interest (GFP) can be targeted an strain by fusion causes normal disrupted. findings underlying implications host interactions use biotechnology discussed.